Separation and purification of hydrolyzable tannin from Geranium wilfordii Maxim by reversed-phase and normal-phase high-speed counter-current chromatography

✍ Scribed by Dan Liu; Zhiguo Su; Changhai Wang; Ming Gu; Siliang Xing

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 232 KB
• Volume: 33
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200900599

✦ Synopsis

Abstract

Three hydrolyzable tannins, geraniin, corilagin and gallic acid, main active components of Geranium wilfordii Maxim, have been separated and purified in one‐step by both reversed‐phase and normal‐phase high‐speed counter‐current chromatography. Gallic acid, corilagin and geraniin were purified from 70% aqueous acetone extract of G. wilfordii Maxim with solvent system n‐hexane–ethyl acetate–methanol–acetic acid–water (1:10:0.2:0.2:20) by reversed‐phase high‐speed counter‐current chromatography at purities of 94.2, 91.0 and 91.3%, at yields of 89.3, 82.9 and 91.7%, respectively. Gallic acid, corilagin and geraniin were purified with solvent system n‐hexane–ethyl acetate–methanol–acetic acid–water (0.2:10:2:1:5) by normal‐phase high‐speed counter‐current chromatography at purities of 85.9, 92.2 and 87.6%, at yields of 87.4, 94.6 and 94.3%, respectively. It was successful for both reversed‐phase and normal‐phase high‐speed counter‐current chromatography to separate high‐polarity of low‐molecular‐weight substances.