Separation and determination of epinephrine and dopamine in traditional Chinese medicines by micellar electrokinetic capillary chromatography with laser induced fluorescence detection

✍ Scribed by Yuming Dong; Hongli Chen; Yonglei Chen; Yang Hui; Xingguo Chen; Zhide Hu

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 753 KB
• Volume: 29
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200600073

✦ Synopsis

Abstract

A micellar electrokinetic capillary chromatography method with laser‐induced fluorescence detection was developed for the analysis of epinephrine and dopamine after derivatization with 4‐chloro‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole. The optimum derivatization conditions were: 30 mM sodium borate (pH adjusted to 8.0 with 1.0 M HCl), reaction time 30 min at 60°C. Baseline separation was achieved within 14 min with a running buffer composed of 10 mM sodium borate + 25 mM sodium dodecyl sulfate (pH adjusted to 9.5 with 0.1 M NaOH) and an applied voltage of 15 kV. Good linearity relationships (correlation coefficients: 0.9991 for epinephrine and 0.9985 for dopamine) between peak areas and concentrations of the analytes were obtained. The detection limits and quantification limits for epinephrine and dopamine were 0.0038 mg/L and 0.013 mg/L, and 0.065 mg/L and 0.020 mg/L, respectively. The method was applied to the analysis of the two compounds in two Chinese medicines with recoveries in the range of 92.6–108.7%.