Determination of catecholamines and serotonin by micellar electrokinetic chromatography with laser-induced fluorescence detection

✍ Scribed by Li-Wei Cao

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 161 KB
• Volume: 21
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.809

✦ Synopsis

Abstract

6‐Oxy‐(N‐succinimidyl acetate)‐9‐(2′‐methoxycarbonyl) fluorescein, a new synthesized fluorescent reagent, was established for the first time as a label for the sensitive analysis of catecholamines (CAs) and serotonin (5‐HT) by micellar electrokinetic chromatography (MEKC) with laser‐induced fluorescence detection. After careful study on the derivatization conditions such as pH value, reagent concentration, temperature and reaction time, the labeling reaction was accomplished as quickly as 7 min with stable yield. The separation parameters for the CAs and 5‐HT were also optimized in detail. The derivatives were baseline separated in a running buffer containing 30 mm boric acid and 15 mm sodium dodeculsulfate at pH 9.0. The detection limits ranged from 5 × 10^−10^ to 2 × 10^−9^ m (signal‐to‐noise ratio = 3). The rapid and sensitive method was also applied to the determination of the CAs and 5‐HT of urine samples. Copyright © 2007 John Wiley & Sons, Ltd.