Separation and determination of 2,4-D, dicamba and 2,4,5-T in tobacco by nonaqueous capillary electrophoresis

✍ Scribed by Hongxia Liu; Juanmei Song; Po Han; Yongbo Li; Shusheng Zhang; Huimin Liu; Yangjie Wu

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 696 KB
• Volume: 29
• Category: Article
• ISSN: 1615-9306
• DOI: 10.1002/jssc.200500373

✦ Synopsis

Separation and determination of 2,4-D, dicamba and 2,4,5-T in tobacco by nonaqueous capillary electrophoresis A practical method for residue analysis of 2,4-D, dicamba and 2,4,5-T in baked tobacco leaves has been developed using nonaqueous CE (NACE). The herbicide residues of 2,4-D, dicamba and 2,4,5-T in tobaccos were extracted by ultrasonication with ethyl acetate, followed by a cleanup procedure with gel permeation chromatography. The separation of 2,4-D, dicamba and 2,4,5-T by NACE was optimized based on orthogonal experiment design with four factors at three levels. The optimal NACE condition was established with the running buffer of 40.0 mmol/L ammonium acetate in 90% CH 3 CN (apparent pH 10.2), and the applied voltage of -25 kV over a capillary of 50 lm id646 cm (37.5 cm to the detector window), which gave a baseline separation of 2,4-D, dicamba and 2,4,5-T within 15 min. The LOD were ca. 0.4 -0.6 lg/mL for the three herbicides, whereas the overall recovery ranged from 80.8 to 84.1%. The proposed method has been successfully applied to measure 300 real tobacco samples, and the residue profiles of the three herbicides in tobacco samples were obtained and evaluated.