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Novel nonaqueous capillary electrophoresis separation and determination of bioactive flavone derivatives in Chinese herbs

✍ Scribed by Shengda Qi; Yuqin Li; Shuixian Wu; Xingguo Chen; Zhide Hu

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
442 KB
Volume
28
Category
Article
ISSN
1615-9306

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✦ Synopsis

Abstract

Nonaqueous CE (NACE) coupled to UV detection is described for the separation and determination of bioactive flavone derivatives in Chinese herbs extraction. After optimization of electrophoresis parameters, including the electrolyte nature and the organic solvent composition, a reliable separation of the analytes in an ACN/methanol (60Β :Β 40, v/v) mixture containing 80 mM Tris and 10 mM sodium cholate was performed. The detection was performed at 254 nm. Method performances, including migration time and peak area reproducibility, linearity, sensitivity, and accuracy, were evaluated. The method was applied to determine bioactive flavone derivatives in seven Chinese herbs.

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