Separating field strength, temperature, and pulsing effects in pulsed field electrophoresis
✍ Scribed by Stéphane Mouradian; Robert L. Brumley Jr.; Dr. Lloyd M. Smith
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 705 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
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It has been reported that secondary pulsed field gel (SPFG) electrophoresis can dramatically increase the speed of separation of large DNA molecules without a decrease in resolution (Zhang, T. Y., Smith, C. L., and Cantor, C. R. (1991) Nucleic Acids Res. 19, \(1291-\) 1296). However, our attempts to
## Abstract ## Purpose: To investigate whether scanning at different field strengths and pulse sequences would influence reproducibility of corpus callosum (CC) morphometric measurements as variations in scanning parameters may result in differences in contrast properties between resulting images