Sensitized chemiluminescence determination of fluorouracil in pharmaceutical and biological fluids based on potassium permanganate oxidation in the presence of formaldehyde

✍ Scribed by Hanwen Sun; Liqing Li; Xueyan Chen

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 183 KB
• Volume: 21
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.20143

✦ Synopsis

A novel chemiluminescence (CL) reaction system was established for the anticancer drug fluorouracil (5-FU). We developed a formaldehyde-sensitized CL emission mechanism for 5-FU-KMnO 4 -HCl systems by comparing the fluorescence emission with CL spectra. The illuminant was the singlet-state bimolecular oxygen

produced in the reaction system, and emits CL spectra at 639 nm. The presence of formaldehyde can sensitize CL emission by accelerating the generation of 1 O 2 ( 1 D g ).

The optimum conditions for CL emission were investigated. The relationship between the relative CL intensity and the concentration of the analyte showed good linearity. The determination limit was 3 Â 10 À8 g mL À1 . The relative standard deviation (SD) was 1.8%. The proposed method was applied to the determination of fluorouracil in pharmaceutical preparations and biological fluids, and satisfactory results were obtained. J.