Sensitive, fluorescent detection of hydrazine via derivatization with 2,3-naphthalene dicarboxaldehyde

✍ Scribed by G.E. Collins; S.L. Rose-Pehrsson

• Book ID: 102981666
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 865 KB
• Volume: 284
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80026-h

✦ Synopsis

The derivatization of hydraxine with 2,3-naphthalene dicarboxaldehyde (NDA) followed by fluorescence detection is shown to be an effective method for the selective determination of extremely low concentrations of hydraxine in solution. Kinetic studies of the reaction between hydraxine and NDA indicate that the reaction is first order in both hydraxine and NDA, and is dependent upon an acidic pH (optimum pH = 2.5) for the formation of the fluorescent derivative. The NDA-hydra&e derivative is readily formed (time response < 5 mitt) and is strongly fluorescent in the green (A, = 403 nm and A,, = 500 nm). A linear concentration dependence is observed over a dynamic range from 50 rig/l to 500 rg/l of hydraxine (correlation coefficient, r > 0.9991, with a signal-to-noise ratio of 3:l for 50 rig/l of hydraxine. The selectivity of the NDA reagent is examined with respect to several possible interferents, including ammonia, and is shown to be a highly specific reagent for hydraxine.