Semisynthetic fluorohydrolases prepared by chemical modification of ribonuclease
β Scribed by David E. Albert; Mary B. Douglas; Marcia A. Hintz; Christopher S. Youngen; Melvin H. Keyes
- Book ID
- 103523084
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 947 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0141-0229
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Glycopeptide analogues of ribonuclease S-peptide, des 16-20 [(Gal /3)Thr3]-S-peptide and des 16-20 [(Gal /3)Thr6]-S-peptide, were synthesized by the solid-phase procedure based on the Fmoc chemistry. Reassociation of the synthetic glycopeptides with S-protein yielded enzymatically active, glycosylat
Detailed methods are described for preparation of bovine pancreatic ribonuclease (polyribonucleotide 2-ofigonucleotidotransferase (cyclizing), EC 2.7.7.16 ) crystals of Modifications I and II of quality suitable for X-ray diffraction studies. The reasons for choice of solvent and buffer systems are