Self-Assembled Nanogel of Hydrophobized Dendritic Dextrin for Protein Delivery

✍ Scribed by Yayoi Ozawa; Shin-ichi Sawada; Nobuyuki Morimoto; Kazunari Akiyoshi

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 498 KB
• Volume: 9
• Category: Article
• ISSN: 1616-5187
• DOI: 10.1002/mabi.200800288

✦ Synopsis

Abstract

Highly branched cyclic dextrin derivatives (CH‐CDex) that are partly substituted with cholesterol groups have been synthesized. The CH‐CDex forms monodisperse and stable nanogels with a hydrodynamic radii of ≈10 nm by the self‐assembly of 4–6 CH‐CDex macromolecules in water. The CH‐CDex nanogels spontaneously trap 10–16 molecules of fluorescein isothiocyanate‐labeled insulin (FITC‐Ins). The complex shows high colloidal stability: no dissociation of trapped insulin is observed after at least 1 month in phosphate buffer (0.1 M, pH 8.0). In the presence of bovine serum albumin (BSA, 50 mg · mL^−1^), which is a model blood system, the FITC‐Ins trapped in the nanogels is continuously released (≈20% at 12 h) without burst release. The high‐density nanogel structure derived from the highly branched CDex significantly affects the stability of the nanogel–protein complex.

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