Selectivity optimization of reversed-phase high-performance liquid chromatographic peptide and protein separations by varying bonded-phase functionality
β Scribed by Barry E. Boyes; Douglas G. Walker
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 942 KB
- Volume
- 691
- Category
- Article
- ISSN
- 1873-3778
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The separation of peptides and proteins by reverse-phase high-performance liquid chromatography with cyanopropylsilyl and large-pore propylsilyl supports, together with aqueous trifluoroacetic acid/acetonitrile gradients, was studied. Operating parameters (trifluoroacetic acid concentration, flow ra
Proteins and peptides were separated in the reversed-phase mode on microcolumns packed with nonporous octadecyl-group bonded silica gel with an average particle diameter of 4.5 or 20 pm. Separation columns were prepared from glass-lined stainless steel tubing of 39 or 56-mm x 0.5-mm i.d. An artifici
## Abstract The influence of the nature of typical organic modifiers, used in reversedβphase systems diluted with water, on retention and selectivity of chiral acetylated diamines and acetonitriles possessing pharmacological activity was investigated. Linear, semilogarithmic relationships between t