𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Selectivity differences between alkyl and polar-modified alkyl phases in reversed phase high performance liquid chromatography

✍ Scribed by Lin-Lin Jing; Ru Jiang; Peng Liu; Ping-An Wang; Tian-Yao Shi; Xiao-Li Sun

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
960 KB
Volume
32
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Compared to conventional C18 phases, polar‐modified phases have distinct differences with regards to chromatographic behavior. In the present study, ODS phases and polar‐modified phases were synthesized. The columns containing these new packings demonstrated satisfactory stability under both acidic (pH 1.5) and basic (pH 10) conditions. We evaluated the selectivity differences between alkyl and polar‐modified alkyl RP columns by using a range of neutral analytes. The polar‐modified alkyl phases showed excellent peak shapes for almost all compounds. We also compared the selectivity differences between them for separating nucleotides by using 100% aqueous mobile phase and tricyclic antidepressants in the intermediate pH mobile phases. The results demonstrated that polar‐modified phases display a significantly reduced hydrophobic nature and a significantly reduced silanol activity compared to the conventional C18 phases.

📜 SIMILAR VOLUMES

Comparison of retention behavior on poly
Comparison of retention behavior on polymeric resins and an alkyl-bonded silica phase in reversed-phase high-performance liquid chromatography
✍ Susan Pedigo; Larry D. Bowers 📂 Article 📅 1990 🏛 Elsevier Science 🌐 English ⚖ 802 KB

The two most obvious differences between alkyl-bonded silica phases and polystyrene-divinylbenzene resins as reversed-phase chromatographic supports are the aromaticity and the lack of hydrogen bonding ability in the polymeric resin. The effect of these differences on the selectivity for a set of sm

Analysis of selected ionic liquid cation
Analysis of selected ionic liquid cations by ion exchange chromatography and reversed-phase high performance liquid chromatography
✍ Piotr Stepnowski; Wojciech Mrozik 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 1016 KB

## Analysis of selected ionic liquid cations by ion exchange chromatography and reversed-phase high performance liquid chromatography The chromatographic behavior of 8 ionic liquids -7 homologues of 1-alkyl-3-methylimidazolium and 4-methyl-N-butylpyridinium -has been investigated with a strong cati