Comparison of retention behavior on polymeric resins and an alkyl-bonded silica phase in reversed-phase high-performance liquid chromatography

✍ Scribed by Susan Pedigo; Larry D. Bowers

• Publisher: Elsevier Science
• Year: 1990
• Tongue: English
• Weight: 802 KB
• Volume: 499
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)96979-6

✦ Synopsis

The two most obvious differences between alkyl-bonded silica phases and polystyrene-divinylbenzene resins as reversed-phase chromatographic supports are the aromaticity and the lack of hydrogen bonding ability in the polymeric resin. The effect of these differences on the selectivity for a set of small solutes was studied through the use of a solvatochromic comparison method. For retention on a polymeric phase for mobile phases with the modifiers methanol and acetonitrile, the linear solvation energy relationship indicated an increased dependence on the polarizability/dipolarity of solutes. For the modifier tetrahydrofuran, retention on the polystyreneedivinylbenzene resin was indistinguishable from that on the alkyl-bonded silica phase. The hydrogen bonding ability of a solute was found to play a greater role in retention on alkyl-bonded silica than on the polymeric resin for all three modifiers. Since the mobile phase compositions were chosen such that the Hildebrand solubility parameters were equal, the dependence of retention on molar volume was found to be the same for all mobile phase-stationary phase combinations examined.