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Selective Transient Heteronuclear Cross Relaxation in a Selectively13Cα-Labeled Peptide

✍ Scribed by Peter Allard; Jüri Jarvet; Astrid Gräslund

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
157 KB
Volume
124
Category
Article
ISSN
1090-7807

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✦ Synopsis

A new pulse sequence for the direct measurement of heteroof relaxation rates should be measured. One of the most nuclear cross-relaxation rates is presented. The pulse sequence frequently measured rates is the heteronuclear cross-relaxuses proton detection of transient carbon magnetization with sensiation rate s between an a carbon and an a proton or between tivity-enhanced transfer of magnetization and pulsed field gradian amide nitrogen and an amide proton. This rate is sensitive ents for coherence selection and water suppression. The heteroto the difference of the spectral density at the frequencies nuclear cross-relaxation rate is measured using either nonselective v H / v X and v H 0 v X and probes the dynamics on the or selective inversion of all or particular protons. A technique for nanosecond time scale. The cross-relaxation rate is usually the suppression of spin diffusion is also applied. The pulse secalculated indirectly from the measured heteronuclear quence is tested on a peptide with a selectively 13 C-labeled asteady-state NOE and the longitudinal-relaxation rate of the carbon. The directly measured rate is compared with and agrees heteronucleus. Here we present a direct and selective method well with the cross-relaxation rates as traditionally calculated from the steady-state NOE and carbon longitudinal-relaxation rate. for measuring the cross-relaxation rate between a hetero-᭧ 1997 Academic Press nucleus and any of its neighboring protons. The basic idea of this experiment is the same as that described by Solomon (4). The sensitivity of the pulse sequence described here is increased by proton detection of the heteronucleus magneti-

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