Selective recognition of CG interruption by 2′,4′-BNA having 1-isoquinolone as a nucleobase in a pyrimidine motif triplex formation

✍ Scribed by Yoshiyuki Hari; Satoshi Obika; Mitsuaki Sekiguchi; Takeshi Imanishi

• Book ID: 104205400
• Publisher: Elsevier Science
• Year: 2003
• Tongue: French
• Weight: 197 KB
• Volume: 59
• Category: Article
• ISSN: 0040-4020
• DOI: 10.1016/s0040-4020(03)00728-2

✦ Synopsis

To develop a novel nucleoside analogue for the effective recognition of CG interruption in a homopurine -homopyrimidine tract of double-stranded DNA (dsDNA), we succeeded in the synthesis of a triplex-forming oligonucleotide (TFO) containing a novel 2 0 ,4 0 -BNA (Q B ) bearing 1-isoquinolone as a nucleobase, and the triplex-forming ability and sequence-selectivity of the TFO (TFO-Q B ) were examined. On melting temperature (T m ) measurements, it was found that the TFO-Q B formed a stable triplex DNA in a highly sequence-selective manner under near physiological conditions.