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Selective reactivity of rhodanese sulfhydryl groups with 5,5′-dithio-bis(2-nitrobenzoic acid)

✍ Scribed by Bernardo Pensa; Mara Costa; Laura Pecci; Carlo Cannella; Doriano Cavallini


Book ID
115734489
Publisher
Elsevier Science
Year
1977
Weight
437 KB
Volume
484
Category
Article
ISSN
0005-2744

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Estimation of protein sulfhydryl groups
✍ Peter M. Steinert; B.Suren Baliga; Hamish N. Munro 📂 Article 📅 1974 🏛 Elsevier Science 🌐 English ⚖ 623 KB

Labeled dithio-bis(2-nitrobenzoate) has been prepared and its usefulness for the measurement of protein sulfhq-dry1 groups has been investigated. The technique is at least 20 times more sensitive than whf~n dithio-bis(2nitrobenzoate) is used calorimetrically. The adduct formed between the reagent an