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Estimation of protein sulfhydryl groups with 5,5′-[35S] dithio-bis(2-nitrobenzoate)

✍ Scribed by Peter M. Steinert; B.Suren Baliga; Hamish N. Munro

Book ID: 102982115
Publisher: Elsevier Science
Year: 1974
Tongue: English
Weight: 623 KB
Volume: 59
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(74)90294-2

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✦ Synopsis

Labeled dithio-bis(2-nitrobenzoate) has been prepared and its usefulness for the measurement of protein sulfhq-dry1 groups has been investigated. The technique is at least 20 times more sensitive than whf~n dithio-bis(2nitrobenzoate) is used calorimetrically. The adduct formed between the reagent and sulfhydrvl groups is stable between pH 3-9 which should enable use of the reagent for the tagging of sulfhydryl-containing proteins in n wide variety of biochemical proredures. Removal of the ndduct from a protein is achieved simply by t.reatment, with 3 thiol reagent. with concomitant restoration of enzymic activitp of the protein. The procedure has hcen successfully tested on alcohol dehgdrogcnase and mammalian I ibosomcs.

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