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Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression

✍ Scribed by Linser, Rasmus; Gelev, Vladimir; Hagn, Franz; Arthanari, Haribabu; Hyberts, Sven G.; Wagner, Gerhard


Book ID
126653491
Publisher
American Chemical Society
Year
2014
Tongue
English
Weight
769 KB
Volume
136
Category
Article
ISSN
0002-7863

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Isotope labelling is a very powerful tool in NMR studies of proteins and has been employed in various ways for over 40 years. 15N and 13C incorporation, using recombinant expression systems, is now commonplace because heteronuclear experiments assist with the fundamental problems of peak resolution