Selective isolation of G-quadruplexes by affinity chromatography
β Scribed by Tianjun Chang; Xiangjun Liu; Xiaohong Cheng; Cui Qi; Hongcheng Mei; Dihua Shangguan
- Book ID
- 116362652
- Publisher
- Elsevier Science
- Year
- 2012
- Tongue
- English
- Weight
- 752 KB
- Volume
- 1246
- Category
- Article
- ISSN
- 1873-3778
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A method for isolating picomole quantities of nascent mercurated DNA from a mixture of cellular nucleic acids using affinity chromatography on thiol-agarose is described. Analysis of mercurated DNA (HgDNA) isolated in the presence of in vivo-labeled cellular RNA or in vitro-synthesized RNA showed a
An affinity adsorbent for ADP-ribosyltransferase (EC 2.4.2.30) has been synthesized by coupling 3-aminobenzamide to Sepharose 4B. Using this material, ADP-ribosyltransferase from human placenta has been purified from crude extract to homogeneity within a few hours. The enzyme has an apparent Km for