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Isolation of Phosphoglycerate Kinases by Affinity Chromatography

✍ Scribed by Günter W. K. KUNTZ; Stefan EBER; Waltraud KESSLER; Helgard KRIETSCH; Wolfgang K. G. KRIETSCH


Book ID
115116634
Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
862 KB
Volume
85
Category
Article
ISSN
1432-1327

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Purification of human erythrocyte phosph
✍ Josiane Chen-Marotel; Yves Blouquit; Raymonde Rosa; Marie-Claude Calvin 📂 Article 📅 1983 🏛 Elsevier Science 🌐 English ⚖ 906 KB

A new method for the purification of human erythrocyte phosphoglyceratekinase involving affinity chromatography on dye-ligand media (Red A), in the presence of 3-phosphoglycerate and ATP, is described. The method is rapid and technically simple. The purity of the enzyme was verified by electrophores