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Affinity chromatography of phosphoglycerate kinases from different sources

✍ Scribed by G. W. K. Kuntz; W. K. G. Krietsch

Book ID
112332856
Publisher
Springer
Year
1978
Tongue
English
Weight
81 KB
Volume
290
Category
Article
ISSN
1618-2650

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Purification of human erythrocyte phosph
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✍ Josiane Chen-Marotel; Yves Blouquit; Raymonde Rosa; Marie-Claude Calvin πŸ“‚ Article πŸ“… 1983 πŸ› Elsevier Science 🌐 English βš– 906 KB

A new method for the purification of human erythrocyte phosphoglyceratekinase involving affinity chromatography on dye-ligand media (Red A), in the presence of 3-phosphoglycerate and ATP, is described. The method is rapid and technically simple. The purity of the enzyme was verified by electrophores