✦ LIBER ✦

Selective inhibition of DNA synthesis by a protein released from spleen cells

✍ Scribed by Shohei Miyata; Hirozi K. Kihara

Publisher: John Wiley and Sons
Year: 1982
Tongue: English
Weight: 324 KB
Volume: 110
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041100316

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Spleen cells cultured release proteins into the culture medium, some of which shows both binding activity to single stranded DNA (ss‐DNA) and inhibitory activity to ^3^H‐thymidine incorporation into cells. The inhibitory protein of DNA synthesis was purified to near homogeneity by ammonium sulfate precipitation, ss‐DNA‐cellulose affinity chromatography, and gel filtration chromatography. The molecular weight of the major band of this protein, as estimated by sodium dodecyl‐sulfate gel electrophoresis, was approximately 14,000. The effect of the protein was compared with various cell lines and primary cell culture, and it was found that the protein strongly inhibited DNA synthesis of HeLa, L, and HEp2 cells, but only inhibited to a minor extent synthesis of Ehrlich cells and primary culture of mouse embryo.

📜 SIMILAR VOLUMES

Reversible inhibition of DNA, RNA and pr

Reversible inhibition of DNA, RNA and protein synthesis in human cells by lead chloride

✍ Yvette Škreb; Vlasta Habazin-Novak 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 353 KB

Inhibition of DNA synthesis and cell div

Inhibition of DNA synthesis and cell division by a cell surface sialoglycopeptide

✍ Heideh Fattaey; Terry C. Johnson; Hsin-Hwei Chou 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 631 KB

We have isolated and purified a cell surface sialoglycopeptide (SGP) from bovine cerebral cortex cells that previously was shown to be a potent inhibitor of cellular protein synthesis. The following studies were carried out to characterize the potential ability of the SGP to inhibit DNA synthesis an

Genetic control of phytohemagglutinin re

Genetic control of phytohemagglutinin responsiveness by murine spleen cells and correlation between the release of lymphotoxins and dna synthesis

✍ Mireille Donner; D. Vaillier; C. Burg 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 603 KB

## Abstract Response to phytohemagglutinin was investigated in adult mouse spleen cells of congenic strains which differ only at the H‐2 locus. The stimulation of spleen cells was assessed by release of lymphotoxins and DNA synthesis. The results obtained show that the response to PHA markedly var

Effect of a potent selective protein kin

Effect of a potent selective protein kinase C inhibitor on histamine release from rat mast cells

✍ Masao Takei; Koichi Endo 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 221 KB

Differential product release (DPR) of pr

Differential product release (DPR) of proteins from yeast: A new technique for selective product recovery from microbial cells

✍ R.-B. Huang; B. A. Andrews; J. A. Asenjo 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 761 KB

Release of mouse eggs from metaphase arr

Release of mouse eggs from metaphase arrest by protein synthesis inhibition in the absence of a calcium signal or microtubule assembly

✍ Dr. Ruth M. Moses; Douglas Kline 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 914 KB

## Abstract Mouse egg activation, which includes release from meiotic metaphase II arrest, results from fertilization‐induced increase in intracellular calcium concentration ([Ca^2+^]~i~). However, during egg activation caused by exposure to the protein synthesis inhibitor, cycloheximide, [Ca^2+^]~