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Selective Immobilization of Proteins onto Solid Supports through Split-Intein-Mediated Protein Trans-Splicing

โœ Scribed by Youngeun Kwon; Matthew A. Coleman; Julio A. Camarero


Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
309 KB
Volume
45
Category
Article
ISSN
0044-8249

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โœ Belinda M. Lew; Kenneth V. Mills; Henry Paulus ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 119 KB ๐Ÿ‘ 1 views

Protein splicing in trans results in the ligation of two protein or peptide segments linked to appropriate intein fragments. We have characterized the trans-splicing reaction mediated by a naturally expressed, approximately 100-residue N-terminal fragment of the Mycobacterium tuberculosis intein and