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Selective Immobilization of Proteins onto Solid Supports through Split-Intein-Mediated Protein Trans-Splicing

✍ Scribed by Youngeun Kwon; Matthew A. Coleman; Julio A. Camarero


Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
308 KB
Volume
118
Category
Article
ISSN
0044-8249

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Protein splicing in trans results in the ligation of two protein or peptide segments linked to appropriate intein fragments. We have characterized the trans-splicing reaction mediated by a naturally expressed, approximately 100-residue N-terminal fragment of the Mycobacterium tuberculosis intein and