✦ LIBER ✦

Selective Harmonic Optical Microscopy : Differentiating Tissue Types with Second Harmonic Generation

✍ Scribed by Shi-Wei Chu

Publisher: Wiley (John Wiley & Sons)
Year: 2008
Weight: 915 KB
Volume: 10
Category: Article
ISSN: 1439-4243
DOI: 10.1002/imic.200890044

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✦ Synopsis

Differentiating Tissue Types with Second Harmonic Generation

SHG is an important contrast mechanism in optical examination for thick biological tissues. Fibrous proteins, such as myosin and collagen, exhibit biophotonic crystal nature and are dominant SHG harmonophores in vertebrates. Local molecule arrangements strongly affect SHG polarization behavior. Here we demonstrate to distinguish myosin-based muscle fibers from intertwined collagenous perimysium through SHG polarization selection, without complicated staining or sample/image processing required.

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