𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography

✍ Scribed by Bo Zhang; Qianying Sheng; Xiuling Li; Qi Liang; Jingyu Yan; Xinmiao Liang

Book ID
102446638
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
288 KB
Volume
34
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Comprehensive glycoprotein characterization based on mass spectrometry (MS) is challenging because of low concentration of glycopeptides and suppression effect of abundant non‐glycosylated peptides in MS. Therefore, it is vital to enrich glycopeptides before MS analysis. A new method was developed to selectively enrich glycopeptides from complex sample by coupling C18 fractionation with titanium dioxide (TiO~2~) enrichment. The new method allows to selectively enrich N‐linked glycopeptides with various glycan forms and different sequence lengths. Compared with single TiO~2~ method, the established method demonstrated higher glycopeptide selectivity and higher glycosylation heterogeneity coverage. Further application of this method to mixture of non‐glycosylated protein and glycoprotein digests at different levels reveals the feasibility of enrichment of tryptic glycopeptides from simple proteomics samples.

📜 SIMILAR VOLUMES

Extraction of Magnesium from Biological
Extraction of Magnesium from Biological Fluids Using 8-Hydroxyquinoline and Cation-Exchange Chromatography for Isotopic Enrichment Analysis Using Thermal Ionization Mass Spectrometry
✍ N.E. Vieira; A.L. Yergey; S.A. Abrams 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 463 KB

The use of 8-hydroxyquinoline to precipitate magnesium was evaluated as a method for preparing biological samples for isotopic enrichment analysis using thermal ionization mass spectrometry (TIMS). Standard curves in matrices of water, serum, and urine were prepared using varying amounts of \({ }^{2

Use of carbon adsorption traps combined
Use of carbon adsorption traps combined with high resolution gas chromatography - mass spectrometry for the analysis of polar and non-polar C4-C14 hydrocarbons involved in photochemical smog formation
✍ Ciccioli, Paolo ;Cecinato, Angelo ;Brancaleoni, Enzo ;Frattoni, Massimiliano ;Li 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 791 KB

## Abstract A method has been developed for the collection and analysis of polar and non‐polar C~4~‐C~14~ hydrocarbons involved in the formation of photochemical smog. Enrichment of hydrocarbons from both polluted and unpolluted areas has been achieved with three‐stage traps packed with carbon adso

Inter-laboratory comparison of elemental
Inter-laboratory comparison of elemental analysis and gas chromatography combustion isotope ratio mass spectrometry (GC-C-IRMS). Part I: δ13C measurements of selected compounds for the development of an isotopic Grob-test
✍ F. Serra; A. Janeiro; G. Calderone; J. M. Moreno Rojas; C. Rhodes; L. A. Gonthie 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 333 KB 👁 1 views

## Abstract This study was directed towards investigating suitable compounds to be used as stable isotope reference materials for gas chromatography combustion isotope ratio mass spectrometry (GC‐C‐IRMS) calibration. Several compounds were selected from those used in the ‘Grob‐test’ mixture. Oxygen