Selective determination of l-ascorbic acid by a chemiluminescence method with ascorbate oxidase

✍ Scribed by K. Sato; Y. Chiba; S. Tanaka

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 390 KB
• Volume: 277
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85089-3

✦ Synopsis

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The determination of L-ascorbic acid by utilizing lucigenin chemiluminescence with ascorbate oxidase was studied. The dehydroascorbic acid produced by the enzymatic reaction of L-ascorbic acid and ascorbate oxidase was found to be a more effective activator than L-ascorbic acid for the lucigenin chemiluminescence reaction. The optimum pH, temperature and incubation time for the enzymatic reaction were S.7,30°C and 10 min, respectively. The calibration graph was linear over the range 0.14-50 pg ml-'. The correlation coefficient for this line was 0.998. The detection limit (2~) was 0.1 pg ml-' and the relative standard deviation was 10% (n = 5) for a concentration of 0.14 pg ml-'. This method was applied to the determination of L-ascorbic acid in commercially available drugs and health drinks.