Simultaneous multichannel nonlinear imag
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R. Gauderon; P.B. Lukins; C.J.R. Sheppard
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Article
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2001
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Elsevier Science
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English
β 241 KB
Simultaneous two-photon excited Β―uorescence (TPF) and second-harmonic generation (SHG) imaging is demonstrated using a single femtosecond laser and a scanning microscope. This composite nonlinear microscopic technique was applied to imaging DNA and chromosomes, and it was shown that the two differen