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Combined Raman and continuous-wave-excited two-photon fluorescence cell imaging

✍ Scribed by Uzunbajakava, Natallia; Otto, Cees

Book ID
115426244
Publisher
Optical Society of America
Year
2003
Tongue
English
Weight
220 KB
Volume
28
Category
Article
ISSN
0146-9592

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Simultaneous two-photon excited Β―uorescence (TPF) and second-harmonic generation (SHG) imaging is demonstrated using a single femtosecond laser and a scanning microscope. This composite nonlinear microscopic technique was applied to imaging DNA and chromosomes, and it was shown that the two differen