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SELECTIVE CLEAVAGE OF THE METHIONYL PEPTIDE BONDS IN RIBONUCLEASE WITH CYANOGEN BROMIDE 1

โœ Scribed by Gross, Erhard; Witkop, Bernhard


Book ID
127386170
Publisher
American Chemical Society
Year
1961
Tongue
English
Weight
231 KB
Volume
83
Category
Article
ISSN
0002-7863

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Enhancement of Cyanogen Bromide Cleavage
โœ Raymond Kaiser; Lorraine Metzka ๐Ÿ“‚ Article ๐Ÿ“… 1999 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 85 KB

Cyanogen bromide (CNBr) is a common chemical used to hydrolyze peptide bonds C-terminal to methionine residues in peptides and proteins. In most cases, the efficiency of this bond cleavage is greater than 90% except in situations where a serine or threonine residue follows methionine in the amino ac