Selective and extensive 13C labeling of a membrane protein for solid-state NMR investigations
β Scribed by M. Hong; K. Jakes
- Book ID
- 110262331
- Publisher
- Springer Netherlands
- Year
- 1999
- Tongue
- English
- Weight
- 60 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0925-2738
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π SIMILAR VOLUMES
We describe an approach to efficiently determine the backbone conformation of solid proteins that utilizes selective and extensive (13)C labeling in conjunction with two-dimensional magic-angle-spinning NMR. The selective (13)C labeling approach aims to reduce line broadening and other multispin com
Solid-state 13C CP/MAS NMR spectra are reported for di β erent EDTA-metal complexes. The e β ect of metal binding on the isotropic 13C chemical shift and principal elements of the carboxyl 13C chemical shift anisotropy (CSA) tensor are discussed.
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