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Assigning large proteins in the solid state: a MAS NMR resonance assignment strategy using selectively and extensively13C-labelled proteins

✍ Scribed by Victoria A. Higman; Jeremy Flinders; Matthias Hiller; Stefan Jehle; Stefan Markovic; Sebastian Fiedler; Barth-Jan van Rossum; Hartmut Oschkinat


Publisher
Springer Netherlands
Year
2009
Tongue
English
Weight
845 KB
Volume
44
Category
Article
ISSN
0925-2738

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We describe an approach to efficiently determine the backbone conformation of solid proteins that utilizes selective and extensive (13)C labeling in conjunction with two-dimensional magic-angle-spinning NMR. The selective (13)C labeling approach aims to reduce line broadening and other multispin com