Selection of paraquat-resistant variants of tobacco from cell cultures
β Scribed by Ouida Kimbrough Miller; Karen W. Hughes
- Book ID
- 112798934
- Publisher
- Society for In Vitro Biology
- Year
- 1980
- Tongue
- English
- Weight
- 478 KB
- Volume
- 16
- Category
- Article
- ISSN
- 1475-2689
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The goal of this work was to begin a genetic study of the molecular mode of action of abscisic acid (ABA), by isolating variant cultured cells resistant to the hormone, or to a factor which induces ABA synthesis, namely water stress. Cell cultures of Nicotiana tabacum L. cv. Wisconsin 38 and N. silv
Variant clones were isolated from Nicotiana silvestris Speg. et Comes cell cultures at low frequencies following severe abscisic-acid (ABA) or mannitol-induced water-stress treatments of plated cells. N. tabacum L. variants were not recovered. Variants from the ABA selection experiments exhibited a