Selection of atrazine-resistant tobacco variants from photomixotrophic cultures
โ Scribed by Wen-Chung Wang; J.R. Myers; G.B. Collins
- Book ID
- 119136634
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 884 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0168-9452
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The goal of this work was to begin a genetic study of the molecular mode of action of abscisic acid (ABA), by isolating variant cultured cells resistant to the hormone, or to a factor which induces ABA synthesis, namely water stress. Cell cultures of Nicotiana tabacum L. cv. Wisconsin 38 and N. silv
Variant clones were isolated from Nicotiana silvestris Speg. et Comes cell cultures at low frequencies following severe abscisic-acid (ABA) or mannitol-induced water-stress treatments of plated cells. N. tabacum L. variants were not recovered. Variants from the ABA selection experiments exhibited a
A mutant cell line that shows high resistance to the photosynthesis-inhibiting herbicide atrazine was selected from cultured photomixotrophic Nicotiana tabacum cv. Samsun NN cells by repeated exposure to toxic levels of the herbicide. This resistance was confirmed by measurements of Hill reaction ac