Selection of aphidicolin-resistant CHO cells with altered levels of ribonucleotide reductase
β Scribed by Carol L. K. Sabourin; Paul F. Bates; Louis Glatzer; Chia-Cheng Chang; J. E. Trosko; John A. Boezi
- Book ID
- 112563559
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 820 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1572-9931
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## Abstract We describe the isolation and characterization of a Chinese hamster ovary cell line selected for resistance to Nβcarbamoyloxyurea. Using the mammalian cell permeabilization assay developed in our laboratory, a detailed analysis of the target enzyme, ribonucleotide reductase (EC 1.17.4.1
Ribonucleotide reductase consists of 2 protein components frequently called M I and M2. Hydroxyurea specifically inhibi t s DNA synthesis by interacting with the M2 protein and destroying a unique tyrosyl-free radical. We have carried out a molecular and cellular characterization of 2 Chinese hamste