Isolation of hydroxyurea-resistant CHO cells with altered levels of ribonucleotide reductase
β Scribed by William H. Lewis; Jim A. Wright
- Book ID
- 112558790
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 902 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1572-9931
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## Abstract We describe the isolation and characterization of a Chinese hamster ovary cell line selected for resistance to Nβcarbamoyloxyurea. Using the mammalian cell permeabilization assay developed in our laboratory, a detailed analysis of the target enzyme, ribonucleotide reductase (EC 1.17.4.1
Ribonucleotide reductase consists of 2 protein components frequently called M I and M2. Hydroxyurea specifically inhibi t s DNA synthesis by interacting with the M2 protein and destroying a unique tyrosyl-free radical. We have carried out a molecular and cellular characterization of 2 Chinese hamste