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Sedimentation of High Molecular Weight DNA released from Protoplasts of Bacillus subtilis

โœ Scribed by YAMAGISHI, HIDEO


Book ID
109663329
Publisher
Nature Publishing Group
Year
1968
Tongue
English
Weight
230 KB
Volume
219
Category
Article
ISSN
0028-0836

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High frequency transformation of Bacillu
โœ Chang, Shing ;Cohen, Stanley N. ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 449 KB

A highly efficient method for transformation of Bacillus subtilis by plasmid DNA is reported. The procedure, which involves polyethylene glycol-induced DNA uptake by protoplasts and subsequent regeneration of the bacterial cell wall, yields up to 80% transformants with an efficiency of 4 x 10(7) tra