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Sedimentation characteristics of RNA from isolated yeast nuclei

โœ Scribed by W.W. Sillevis Smitt; G. Nanni; Th.H. Rozijn; G.J.M. Toning


Book ID
118875712
Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
546 KB
Volume
59
Category
Article
ISSN
0014-4827

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Rapid isolation of yeast nuclei
โœ Gregory J. Ide; Court A. Saunders ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 557 KB

A procedure has been developed for the rapid isolation of yeast nuclei in high yield using Percoll gradients. The nuclei are substantially free of cytoplasmic contamination as measured by alcohol dehydrogenase activities, have the typical chromatin digestion pattern when digested with nucleases, are