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Rapid isolation of yeast nuclei

✍ Scribed by Gregory J. Ide; Court A. Saunders

Publisher: Springer-Verlag
Year: 1981
Tongue: English
Weight: 557 KB
Volume: 4
Category: Article
ISSN: 0172-8083
DOI: 10.1007/bf00365686

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✦ Synopsis

A procedure has been developed for the rapid isolation of yeast nuclei in high yield using Percoll gradients. The nuclei are substantially free of cytoplasmic contamination as measured by alcohol dehydrogenase activities, have the typical chromatin digestion pattern when digested with nucleases, are useful for isolation of nuclear proteins and for in vitro transcription experiments.

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