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Secretion of recombinant ribonuclease T1 into the periplasmic space of Escherichia coli with the aid of the signal peptide of alkaline phosphatase

✍ Scribed by Takao Fujimura; Toshiki Tanaka; Kanako Ohara; Hiroshi Morioka; Seiichi Uesugi; Mono Ikehara; Satoshi Nishikawa


Book ID
115923695
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
632 KB
Volume
265
Category
Article
ISSN
0014-5793

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Recombinant plasmids with genes for the
✍ Boidol, Werner ;Simonis, Marianne ;TΓΆpert, Michael ;Siewert, Gerhard πŸ“‚ Article πŸ“… 1982 πŸ› Springer 🌐 English βš– 304 KB

Restriction maps of several recombinant plasmids representing a section of the E. coli K12 chromosome 35,000 bp in size with the genes phoA, proC and phoB were prepared. The orientation of phoA and the exact position of its N-terminal end on this map were determined by identifying a subfragment whic