Secretion of Biologically Active Human Granulocyte Colony-Stimulating Factor (G-CSF) in Milk of Transgenic Mice
โ Scribed by G. A. Dvoryanchikov; I. A. Serova; L. E. Andreeva; L. P. B. Dias; S. Azevedo; O. L. Serov
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2005
- Tongue
- English
- Weight
- 101 KB
- Volume
- 41
- Category
- Article
- ISSN
- 1022-7954
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A chemiluminescence sandwich enzyme immunoassay, using a glucose oxidase (GO) label, was developed for detecting attomole amounts o f human granulocyte colony stimulating factor (G-CSF). Purified goat F(ab')\* immobilized on a bead and purified goat Fab' labelled with GO were selected in combination
Combinations of low dosages of purified recombinant human (rh) macrophagecolony stimulating factor (M-CSF; also termed CSF-1) and rh granulocyte-colony stimulating factor (G-CSF) were compared alone and in combination for their influence on the cycling rates and numbers of bone marrow and splenic gr