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Second harmonic detection of sinusoidally modulated two-photon excited fluorescence

✍ Scribed by Freeman, R. Griffith.; Gilliland, Donald L.; Lytle, Fred E.

Book ID
126186817
Publisher
American Chemical Society
Year
1990
Tongue
English
Weight
541 KB
Volume
62
Category
Article
ISSN
0003-2700

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Simultaneous two-photon excited Β―uorescence (TPF) and second-harmonic generation (SHG) imaging is demonstrated using a single femtosecond laser and a scanning microscope. This composite nonlinear microscopic technique was applied to imaging DNA and chromosomes, and it was shown that the two differen