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Screening of large protein libraries by the “cell immobilized on adsorbed bead” approach

✍ Scribed by Amihay Freeman; Noa Cohen-Hadar; Simona Abramov; Ronit Modai-Hod; Yael Dror; George Georgiou


Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
773 KB
Volume
86
Category
Article
ISSN
0006-3592

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✍ Rainer Gast; Jörn Glökler; Maria Höxter; Michael Kieß; Ronald Frank; Werner Tegg 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 258 KB

A method is described for the elucidation of the peptide substrate phosphorylation specificity of a protein kinase. Peptide libraries with two to six degenerate positions and a length of seven or nine amino acids were generated directly on Sepharose beads by solidphase peptide synthesis according to