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Screening of effective column rinse solvent for Protein-A chromatography

โœ Scribed by Ryosuke Yumioka; Kouhei Tsumoto; Tsutomu Arakawa; Daisuke Ejima


Book ID
116829063
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
370 KB
Volume
70
Category
Article
ISSN
1046-5928

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A simple and effective solvent system fo
โœ Shuji Matsuura; Hao-Chia Chen ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 663 KB

Elution of gonadotropins and other glycoproteins from concanavahn A has been previously performed with competitive ligands such as a-methyl-D-mannopyranoside and a-methyl-o-glucopyranoside. In comparative studies we found that 1 M NHIOH was a highly effective eluent solution and gave nearly complet