✦ LIBER ✦

A simple and effective solvent system for elution of gonadotropins from concanavalin A affinity chromatography

✍ Scribed by Shuji Matsuura; Hao-Chia Chen

Book ID: 102984408
Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 663 KB
Volume: 106
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90540-0

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✦ Synopsis

Elution of gonadotropins and other glycoproteins from concanavahn A has been previously performed with competitive ligands such as a-methyl-D-mannopyranoside and a-methyl-o-glucopyranoside.

In comparative studies we found that 1 M NHIOH was a highly effective eluent solution and gave nearly complete recovery of gonadotropin as revealed by protein contents, immunological, and biological activities. Advantages of 1 M NH,OH over the sugar derivatives are its low cost and volatile nature, and its ability to irreversibly inactivate concanavalin A. The use of this system permits further steps of purification to be performed directly or after lyophilization without dialysis.

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