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Screening for restriction endonucleases in aerobic, thermophilic eubacteria

✍ Scribed by Sigrídur Hjörleifsdóttir; Sólveig K. Pétursdóttir; Jaana Korpela; Anna-Maria Torsti; Pekka Mattila; Jakob K. Kristjánsson

Book ID
104649417
Publisher
Springer-Verlag
Year
1996
Tongue
English
Weight
411 KB
Volume
10
Category
Article
ISSN
0951-208X

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✦ Synopsis

A total of 216 Icelandic aerobic, heterotrophic, thermophiles belonging to three different genera were screened for type II restriction endonucleases. The frequency of positive strains was 44% for both Thermus and Bacillus but 63% for Rhodothermus. Approximately half of the enzymes from each group were characterised and a total of 14 different restriction enzymes were found. In all cases they were isoschizomers of known enzymes. Thermus contained 9 different types, Bacitlus 6 and Rhodothermus had 3. This is the first time that isoschizomers of BspEI, BglI, EagI and EcoRV are found in Thermus and BstBI and EcoRV are found in Rhodothermus.

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