Screening for renal carcinoma associated mutations in the von Hippel-Lindau tumor suppressor gene by temperature gradient gel electrophoresis
✍ Scribed by Michael Wenzel; Jürgen Enczmann; Markus Uhrberg; Ana Hernández; Ulrich Wiese; Rolf Ackermann; Bernd Schmitz-Draeger; Thomas Ebert; PD; Dr. Peter Wernet
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 616 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0173-0835
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30 cycles, denaturation 94 (1 min) annezling 58 (1 Min.) extension 72 (1 min.), magnesium concentration 1.5mM, electrophoresis: 58 degrees celsius, 16 hours, 80 volts,
Germline mutations in the von Hippel-Lindau (VHL) disease tumor suppressor gene (TSG) convey a high risk of clear-cell renal-cell carcinoma (CC-RCC) and most sporadic CC-RCCs demonstrate somatic inactivation of the VHL TSG. However, the existence of further CC-RCC gatekeeper genes is implied by CC-R