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Screening and characterization of a phosphopentomutase useful for enzymatic production of 2′-deoxyribonucleoside

✍ Scribed by Nobuyuki Horinouchi; Takako Kawano; Takafumi Sakai; Seiichiro Matsumoto; Mie Sasaki; Yoichi Mikami; Jun Ogawa; Sakayu Shimizu

Publisher
Elsevier
Year
2009
Tongue
English
Weight
610 KB
Volume
26
Category
Article
ISSN
1871-6784

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✦ Synopsis

Bacillus sphaericus AKU 229 was found to produce an acetaldehyde-tolerant and phosphorylated compound-tolerant phosphopentomutase useful for enzymatic 2'-deoxyribonucleoside production. The gene encoding the phosphopentomutase was cloned and expressed in Escherichia coli. The E. coli expressing B. sphaericus phosphopentomutase was an excellent catalyst as to production of 2'-deoxyribonucleoside in the presence of acetaldehyde and phosphorylated compounds such as fructose 1,6-diphosphate, and d-glyceraldehyde 3-phosphate, which are derived from glucose through glycolysis with yeast cells, and exist abundantly in the practical reaction mixture for enzymatic 2'-deoxyribonucleoside production.

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