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Scale-up Production of a Recombinant Mycobacterium leprae Antigen

✍ Scribed by MICHAEL J. FRUDE; ANDREA READ; LAWRENCE D. KENNEDY


Book ID
119871099
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
258 KB
Volume
721
Category
Article
ISSN
0890-6564

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We present here one systematic strategy to optimize the preliminag purification of the recombinant l&Da-hsp from Mycobacterium leprae at laboratory level in order to design a scaling up process. In a few steps a pure protein-as determined by western blot-was obtained. The overall process recovered 3