Procedures for scaling up the recombinan
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Maria Helena B. Costa; Celina M. P. M. Ueda; Rony A. Sato; CΓ©lia Liberman; IsaΓa
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Article
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1995
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Springer-Verlag
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English
β 386 KB
We present here one systematic strategy to optimize the preliminag purification of the recombinant l&Da-hsp from Mycobacterium leprae at laboratory level in order to design a scaling up process. In a few steps a pure protein-as determined by western blot-was obtained. The overall process recovered 3