𝔖 Bobbio Scriptorium
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Scale-up of natural product formation and isolation

✍ Scribed by Ralf G. Berger; Thomas Scheper; Karl Schügerl


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
414 KB
Volume
49
Category
Article
ISSN
1613-4125

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The expression of the recombinant wild-type NAD + -and mutant NADP + -dependent formate dehydrogenases (EC 1.2.1.2., FDH) from the methanol-utilizing bacterium Pseudomonas sp. 101 in Escherichia coli cells has been improved to produce active and soluble enzyme up to the level of 50% of total soluble